Project Name : Reactive Oxygen Species and Cancer
Theme Name : Health
The project is based on the ROS stress in cancer cells, its mechanism and relationship with mitochondria malfunction and therapeutic strategies can be followed for the treatment of cancer instead of chemotherapy and other methods which generally have side effects.
Instead of cells, due to practical difficulties [like high cost, fast rate of cells] we adopted the model system of – Chlorella vulgaris.
Gillard‟s f/2 medium – Enriched sea water medium prepared.
Optimum conditions required were:
Temp – 250 Degree C
Florescent white light – 16 hrs.
Dark – 8 hrs
Incubation – 100 RPM
Samples were diluted and calculated using Hemo-cytometer.
DHE dye was used in Fluorescence measurement.
Conclusion:
With the amount of RDS present in a cell it can be determined whether the particular cell is cancerous or not. It needs to be maintained in a narrow optimum bandwidth.
